Mining published lists of cancer related microarray experiments: Identification of a gene expression signature having a critical role in cell-cycle control

BACKGROUND: Routine application of gene expression microarray technology is rapidly producing large amounts of data that necessitate new approaches of analysis. The analysis of a specific microarray experiment profits enormously from cross-comparing to other experiments. This process is generally performed by numerical meta-analysis of published data where the researcher chooses the datasets to be analyzed based on assumptions about the biological relations of published datasets to his own data, thus severely limiting the possibility of finding surprising connections. Here we propose using a repository of published gene lists for the identification of interesting datasets to be subjected to more detailed numerical analysis. RESULTS: We have compiled lists of genes that have been reported as differentially regulated in cancer related microarray studies. We searched these gene lists for statistically significant overlaps with lists of genes regulated by the tumor suppressors p16 and pRB. We identified a highly significant overlap of p16 and pRB target genes with genes regulated by the EWS/FLI fusion protein. Detailed numerical analysis of these data identified two sets of genes with clearly distinct roles in the G1/S and the G2/M phases of the cell cycle, as measured by enrichment of Gene Ontology categories. CONCLUSION: We show that mining of published gene lists in the absence of numerical detail about gene expression levels constitutes a fast, easy to perform, widely applicable, and unbiased route towards the identification of biologically related gene expression microarray datasets

Diffuse glioblastoma resembling acute hemorrhagic leukoencephalitis

We report the case of a young man with sudden onset of diplopia after an upper respiratory tract infection. Based on the first radiological findings acute hemorrhagic leukoencephalitis, a variant of acute disseminated encephalomyelitis, was suspected and treatment with high dose intravenous dexamethasone was started but it was stopped for intolerance. The patient clinically worsened, developing gait instability, ataxia and ophthalmoplegia; brain MRI performed 20 days later showed severe progression of the disease with subependymal dissemination. After brain biopsy of the right temporal lesion the histological diagnosis was glioblastoma. These findings suggest that MRI features of acute hemorrhagic leukoencephalitis may dissimulate the diagnosis of diffuse glioma/glioblastoma. This case underscores the importance of considering diffuse glioma in the differential diagnosis of atypical signs and symptoms of acute hemorrhagic leukoencephalitis and underlines the relevant role of integrating neuroradiologic findings with neuropathology

Giosuè Carducci prosatore

Questo volume su Giosuè Carducci prosatore raccoglie i contributi presentati al XVII Convegno internazionale di Letteratura italiana "Gennaro Barbarisi", tenutosi a Palazzo Feltrinelli (Gargnano del Garda) dal 29 settembre al 1° ottobre 2016. Si è trattato di una proficua occasione di incontro, di studio e di approfondimento su un tema forse poco frequentato, soprattutto in tempi recenti, ma ricco di sollecitazioni per una più articolata e storicamente fondata definizione della personalità di un autore così significativo nel panorama della cultura italiana fra Otto e primo Novecento; non soltanto sul versante della poesia (un primato sancito dal premio Nobel nel 1906) ma anche, e forse ancora di più, su quello della prosa saggistica, degli scritti di polemica, delle curatele editoriali, delle ricerche erudite, fino alle prove di alta oratoria e all'epistolografia

The effects of reimbursement mechanisms on medical technology diffusion in the hospital sector in the Italian NHS

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Access the most recent version at doi: 10.1110/ps.0223603 References

Fishing new proteins in the twilight zone of genomes: The test case of outer membrane proteins in Escherichia coli K12, Escherichia coli O157:H7, and other Gram-negative bacteri

Development of Organizational Citizenship Behavior and the Effect of Psychological Contract Fulfillment

<p><b>Copyright information:</b></p><p>Taken from "Mining published lists of cancer related microarray experiments: Identification of a gene expression signature having a critical role in cell-cycle control"</p><p></p><p>BMC Bioinformatics 2005;6(Suppl 4):S14-S14.</p><p>Published online 1 Dec 2005</p><p>PMCID:PMC1866394.</p><p></p>hment evaluated by GoTM. Analysis of expanded cluster of genes repressed by pRB, p16, and EWS/FLI using, as reference gene list, NCBI Gene identifiers annotated in the Affymetrix HG_U95Av2 platform. Plotted categories show enrichment greater than 5 fold and occur more than 5 times in the expanded cluster. Analysis of expanded cluster of genes repressed by pRB, p16, and EWS/FLI using as reference gene list the two expanded clusters merged. All the significantly enriched categories are plotted Analysis of expanded cluster of genes induced by pRB, and repressed by EWS/FLI using as reference gene list, NCBI Gene identifiers annotated in Affymetrix HG_U95Av2 platform. Only categories enriched more than 5 fold and occuring more than 5 times are represented. Analysis of expanded cluster of genes induced by pRB, and repressed by EWS/FLI using as reference gene list the merger of the two expanded clusters